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单细胞数据分析核心:降维聚类方法的选择与应用
单细胞测序数据天然具有高维度、强噪声、稀疏性和细胞异质性等特点,因此降维成为单细胞分析流程中不可或缺的步骤。降维的核心目标是去除冗余信息、突出生物学差异,并通过低维嵌入为聚类、可视化和后续分析如轨迹推断、细胞鉴定等提供可靠表示。
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