We introduce a method of in vitro recombination or “DNA shuffling” to generate libraries of evolved enzymes. The approach relies on the ordering, trimming, and joining of randomly cleaved parental DNA ...

Small supernumerary marker chromosomes are estimated to occur in 1.5 per 1,000 livebirths. One of the most common chromosome markers is the inv dup (15), which may represent as many as 50% of the ...